Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline liquid, sterile-filtered, DPBS Modified, without calcium, without magnesium, suitable for cell culture | MFCD00131855 | 1000ML
Dulbecco's Phosphate Buffered Saline liquid, sterile-filtered, DPBS Modified, without calcium, without magnesium, suitable for cell culture | MFCD00131855 | 1000ML
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 20 X 1ml
Crude cell extracts typically contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins During cell or tissue protein extraction in vitro protein synthesis is significantly inhibited while degradation is enhanced Adding inhibitors against these enzymes can improve yields of intact proteins Protease inhibitor cocktails are used with cell lysates or tissue extracts to enhance protein stability Our protease inhibitor cocktail has broad specificity and serves as a general-use protease inhibitor set suitable for purifying histidine-tagged proteins It contains multiple critical components including AEBSF Aprotinin Bestatin E-64 Leupeptin Phosphoramidon and Pepstatin A with broad inhibition specificity against serine proteases cysteine proteases acidic proteases aminopeptidases and metalloproteases The product is supplied as a ready-to-use solution in DMSO and can be used in WB Co-IP pull-down assays IF IHC and kinase assays
Apexbio Technology LLC Annexin V-PE Reagent 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes To use this reagent additional purchase of 10X Binding Buffer (Cat No K2284) is required or you can directly purchase the Annexin V-PE Apoptosis Kit which includes the buffer (Cat No K2281) To further identify different stages of apoptosis we provide ready-to-use double-staining kits Annexin V-PE/7-AAD Apoptosis Kit (K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 6X1L
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 6X1L
Research Products International Corp PBS 1X SOLN 1ML TUBES 36/CS
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 1.0 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 4.5 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 1.0 ml of PBS per tube
Sterile
36 x 1.0 ml
Bioworld Carbonate-Buffered Saline, pH 9.0 (5X), 250 mL
Product DescriptionCarbonate-buffered saline contains 125mM sodium carbonate and 750mM sodium chloride, at a pH 9.0. This buffer has a concentration of 5X. Carbonate buffering system is important in different cellular processes such as its use to maintain the pH level of mammalian blood, the formation of acid in the lumen of the stomach, and to neutralize the pH of the chyme.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Biocare Medical, LLC PBS Plus, 10X
For laboratory use and procedures requiring a high quality phosphate buffered saline with superior pH stability. PBS Plus (sodium azide free) buffer works well for manual and automated histopathology and immunohistochemistry (IHC) applications with the addition of a surfactant.
Enterprise Technology Solutions Expanding File Jan-Dec, 9" Expansion, 12 Sections, 1/12-Cut Tab, Letter Size, Brown, Elastic Cord
ABILITYONE 7530016819290 Skilcraft Increase your efficiency with this letter-size expanding file featuring 12 tabs for monthly filing. The accordion-style pocket folder is covered with soil-resitant leather-patterned paper and is durable to stand up to heavy handling.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959194 10X ADJ BUF 1.0M NACL .5M THCL