Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6) - 500ml
HEPES Buffer (1 M pH 7 6) - 500ml (For Research Use and Further Manufacturing Only)
Teknova 5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile. 4 pack.
445mM Tris, 445mM Boric Acid, 10mM Ethylenediaminetetraacetic acid (EDTA)
Teknova 10X TBS, pH 7.4. (247mM Tris, 1.37M NaCl) 1000mL, Sterile. 2 pack.
247mM Tris-HCl,1.37M Sodium Chloride
Teknova 20X PBS Solution with 0.1% Tween-20. 1000 mL, Non-Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 10X TE Buffer Solution. 250mL, DNase/RNase, PCR Grade, Sterile. 4 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Alkali Scientific CellPro™ PBS 10X, pH 7.2, without calcium, magnesium, 500mL, 500ml
CellPro™ PBS, 10X, pH7.2, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Rockland Immunochemicals 1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED - MB-040
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. Tris-EDTA buffer is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
ILEX LIFE SCIENCES LLC TRYPSIN 10X P10-022100 100ML
PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 2.50% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Rockland Immunochemicals 1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED - MB-058
1X TES pH 7.8 DEPC TREATED - TES buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. TES buffer is useful to stabilize proteins, DNA, or RNA, while protecting it from degradation.
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt | 71119-22-7 | MFCD00064350 | 100 g
MOPS sodium salt | Purity: ≥99.5% | Mol Wt: 231.25 | 71119-22-7 | MFCD00064350 | 100 g
Enzo Life Sciences Physiological saline (NaCl 0.9%) (endotoxin-free) (sterile) (1.5ml)
Sterile and endotoxin-free physiological saline for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
Electron Microscopy Sciences Perfusion Wash, Technical Grade, 7.2-7.4 pH 1000 ML
For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality. Perfusion wash and fixative technical grade. To be used for standard stains. Phosphate Buffered Saline 01.M Phosphate Buffer pH 7.2-7.4.
Grade: Technical
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-500.02.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7