Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Tris-Glycine Native Sample Buffer (4X), 25 ml
Tris-Glycine Native Sample Buffer (4X), 25 ml.
Biotang Inc Phosphate Buffered RIPA with Glycerol (2X), 125 ml
Phosphate Buffered RIPA with Glycerol (2X), 125 ml.
Active Motif TE, pH 8.0
Active Motif’s TE, pH 8.0 is a commonly used buffer solution in molecular biology. TE buffer can be used for a variety of applications including solubility of DNA or RNA solutions. TE buffer is also used to wash Protein G agarose beads prior to use in immunoprecipitation. TE buffer is 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 and is provided ready to use.
![eMolecules tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | 1365570-27-9 | MFCD22415193 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502157040.JPG-250.jpg)
eMolecules tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | 1365570-27-9 | MFCD22415193 | 1g
Pharmablock | tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | 1g | 551087381 | PB116470 | | 1365570-27-9 | MFCD22415193 | 241.331 | C13H23NO3
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Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 1kg
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culturePurity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 1kg
Sigma Aldrich Fine Chemicals Biosciences HEPES >=99.5% (titration) | 7365-45-9 | MFCD00006158 | 5KG
HEPES >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 5KG
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 25g
HEPES | Purity: 99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 25g
Medchemexpress LLC MES sodium salt | 71119-23-8 | 98.0% | 50 G
MES sodium salt | 71119-23-8 | 98.0% | 50 G
Cell Signaling Technology Tris-Glycine SDS Running Buffer (10X) 1000 ml
Tris-Glycine SDS Running Buffer (10X) 1000 ml
Zymo Research Corporation Endo-Wash Buffer, 120 ml
Endo-Wash Buffer (120 ml). For use in Zyppy™ Plasmid Purification Kits. Sold individually or as a part of the Zyppy™ plasmid purification product line from Zymo Research.