Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255318.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Bioworld ACES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 250 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255311.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 250 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 100 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5
Bioworld BES, 1M, pH 7.0, 100 mL
BES bufferAlso known as N,N-Bis(hydroxyethyl)-2aminoethanesulfonic acid.Technical Information:pKA (25 C): 7.1Heavy Metals: <0.0005%. Appearance: LiquidBoiling Point : 404.34 °C (Predicted)Density : 1.44 g/cm3 (Predicted)Melting Point: 152-154 °CPH Value: 6.4-7.8
Bioworld Bicine, 500 g
A zwitterionic amino acid buffer agent used for low temperature work Technical Information:Synonym: N,N-Bis(2-hydroxyethyl)glycine Titration: > 98.0% (0.1N NaOH)Water content: < 2.0%Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.Appearance: PowderBoiling Point : 388.55 °C at 760 mmHgDensity : 1.05 g/cm3 at 20 °C (lit.)HS Code : 29225000Melting Point: 190 °C (dec.)(lit.)PH Value: 4.3-5.3 (1%)RTECS Number: MB9700000
Teknova 100mM Tris-HCl, pH 8.2. 1000mL, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
Bioworld ADA Buffer, 0.2M, pH 6.0, 500 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld Tris Buffered Saline Packs, 40 PK(s)
Tris Bufferd Saline Packs An ideal all-purpose binding buffer.Each pack yields 500 ml of 25 mM Tris, 0.15 M NaCl, pH 7.2 when dissolved in 500 ml deionized water (total 5 liters). Quantity: 10 packs
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661398.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are solid tablets designed for easy dissolution in water, making it simple to prepare 200mL of phosphate-buffered saline. These tablets are ideal for laboratories that frequently need to prepare large volumes of PBS for use in cell culture, microbiology, and molecular biology research. The tablets dissolve quickly and efficiently, ensuring that the resulting solution maintains the proper pH and osmolarity for reliable experimental results. Each bottle contains 100 tablets, providing an economical and convenient way to prepare PBS without the need for precise measuring or mixing of individual components.